For general questions please contact our Customer Service:
EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: emdinfo@emdchemicals.com
30 July 2010
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A sensitive and high speed homogeneous assay for detection of enzyme activity and for screening of Akt1/PKBα (Protein Kinase B alpha) inhibitors in a high-throughput screening (HTS) format. This kit is based on a fluorescence superquenching technology that does not require antibodies or radioactive label.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Fluorometric | ||
| Sensitivity | 25 pM | ||
| Assay time | 2 h | ||
| Sample type | Recombinant or purified enzyme | ||
| Kit contains | Akt1/PKBalpha Substrate, Akt1/PKBalpha Calibrator, Akt1/PKBalpha Assay Buffer, 10X Sensor Concentrate, Sensor Dilution Buffer | ||
| Declaration |
Sold under license of U.S. Patents 6,589,731 and 6,743,640 |
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| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue or Dry Ice
Multiple Toxicity Values, refer to MSDS |
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| Data | |||
|---|---|---|---|
 Schematic Representation of the Calbiochem<sup>®</sup> TruLight™ Assay. The TruLight assays are based on a proprietary technology utilizing microspheres that are coated with fluorescent polymers and a metal ion coordination complex. Peptide substrates are labeled with a quencher that will quench fluorescence when bound. When the peptide becomes phosphorylated, it binds the microsphere, resulting in fluorescence quenching and a decrease in the fluorescent signal. |
