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70554  Streptavidin AP LumiBlot™ Kit

Chemiluminescent blot detection of biotinylated proteins and nucleic acids

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09 February 2012

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70554-3  25 blot 
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Prices are subject to change without notice.
The Streptavidin AP LumiBlot™ Kit is designed for convenient, sensitive detection of immobilized biotinylated proteins and other macromolecules by chemiluminescence. Detection is based on the binding of streptavidin-alkaline phosphatase conjugate (Streptavidin AP) to the biotinylated target molecule, followed by development with CDP-Star® substrate. The chemiluminescent signal can be detected by exposure to standard x-ray film and is also compatible with standard instrumentation having chemiluminescent detection capability. Kit reagents and protocol are optimized for high signal and minimal nonspecific background in Western blot applications. Under the conditions recommended in the kit, less than 1 ng of biotinylated protein can be detected in a single band on Western blots.
Product information
Avoid freeze/thaw Yes
Components
50 µlStreptavidin AP Conjugate
3 × 200 ml20X TBST
40 ml10% Gelatin
40 ml CDP-Star Substrate (includes Nitro-Block II™ signal enhancer)
pkg/25gLOCATOR Luminescent Labels
pkg/25Development Folders
Store and ship information
Storage +2°C to +8°C
Ship Blue Ice Only
Multiple Toxicity Values, refer to MSDS

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© Merck KGaA, Darmstadt, Germany, 2012

All references to Merck refer to Merck KGaA, Darmstadt, Germany.

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