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Strep•Tag® II Perfect Protein™ Markers, 16–100 kDa, Lyophilized
Accurate, convenient molecular weight determination by SDS-PAGE and immunblotting
For general questions please contact our Customer Service:
EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: Customer.CareDesk@emdchemicals.com
11 February 2012
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The Perfect Protein™ Markers are a novel set of recombinant proteins with defined
sizes at convenient intervals. Designed for routine use in SDS-polyacrylamide gel
electrophoresis, the Perfect Protein Markers enable highly accurate size determination of
unknown samples. Unlike many conventional markers (e.g., ovalbumin, serum albumin, etc.), the Perfect Protein Markers contain no oligosaccharides that cause anomalous migration, heterogeneous "fuzzy" bands, or inaccurate size estimation. The markers are optimized for use with Coomassie blue staining, but adjusted amounts can also be used with other gel staining methods (e.g., silver staining, fluorescent dyes, etc.).
The Strep•Tag® II Perfect Protein™ markers, 16-100kDA include protein sizes of 16, 23.5, 30, 45 60, and 100 kDA. Each marker contains the Strep•Tag II sequence and can be detected by the Strep•Tag II Monoclonal Antibody (Cat. No. 71590) in Western blots. They are provided lyophilized and are supplied with a Strep•Tag II Marker Reconstitution Buffer which also serves as the loading buffer.
The 4X SDS Sample Buffer (Cat. No. 70607), which is separately available, is a standard formulation commonly used for SDS-PAGE analysis of proteins. The solution includes DTT for complete denaturation of disulfide bonds. The buffer can be used at a final concentration of 2X for most applications.
The Strep•Tag® II Perfect Protein™ markers, 16-100kDA include protein sizes of 16, 23.5, 30, 45 60, and 100 kDA. Each marker contains the Strep•Tag II sequence and can be detected by the Strep•Tag II Monoclonal Antibody (Cat. No. 71590) in Western blots. They are provided lyophilized and are supplied with a Strep•Tag II Marker Reconstitution Buffer which also serves as the loading buffer.
The 4X SDS Sample Buffer (Cat. No. 70607), which is separately available, is a standard formulation commonly used for SDS-PAGE analysis of proteins. The solution includes DTT for complete denaturation of disulfide bonds. The buffer can be used at a final concentration of 2X for most applications.
| Related products | |
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| 69079 | Perfect Protein™ Markers, 10-225 kDa |
| 69149 | Perfect Protein™ Markers, 15-150 kDa |
| Related products for Strep•Tag® II Perfect Protein™ Markers, 16–100 kDa, Lyophilized | |
| 69079 | Perfect Protein™ Markers, 10-225 kDa |
| 69149 | Perfect Protein™ Markers, 15-150 kDa |
| Product information | |||
|---|---|---|---|
| Features and benefits |
• Markers are supplied at the working dilution in
gel loading buffer; concentration optimized for
Coomassie blue staining • Recombinant, unmodified markers produce sharp, accurately sized bands |
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| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Standard Handling |
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