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70851  SpinPrep™ Plasmid Kit

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04 February 2012

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70851-3  100 rxn  This Product is currently discontinued or not available.
 
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The SpinPrep™ Plasmid Kit is designed to purify up to 20 µg plasmid DNA from 1 to 3 ml bacterial cultures using small spin columns with silica membranes.

Following alkaline lysis, the cleared lysate is applied to a spin cartridge and the plasmid DNA is adsorbed to the membrane in the presence of chaotropic salt. After a brief wash, the plasmid DNA is eluted in a small volume of low-salt buffer.


Product information
Components
0.1 mlRNase A Solution
10 mlBacterial Resuspension Buffer
20 mlBacterial Lysis Buffer
27 mlSpinPrep Wash Buffer
10 mlSpinPrep Elute Buffer
40 mlSpinPrep Neutralization Buffer
100SpinPrep Filters
100Receiver Tubes
100Eluate Receiver Tubes
Features and benefits
  • No organic extraction or alcohol precipitation
  • Plasmid DNA can be used directly for restriction digestion, labeling, sequencing, transformation, PCR
  • Purified plasmid free of visible contaminants (e.g., genomic DNA, RNA)
  • A260:A280 ratio 1.6–2.0
  • Total preparation time < 30 minutes
Store and ship information
Storage +15°C to +30°C
Ship Shipped at Ambient Temperature or with Blue Ice
Multiple Toxicity Values, refer to MSDS

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© Merck KGaA, Darmstadt, Germany, 2012

All references to Merck refer to Merck KGaA, Darmstadt, Germany.

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