71438
ProteoEnrich™ ATP-Binders™ Kit
Affinity enrichment of ATP-binding proteins
For general questions please contact our Customer Service:
EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: Customer.CareDesk@emdchemicals.com
09 February 2012
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This product has been discontinued.
We apologize for the inconvenience, but we do not currently have an alternative product.
The ProteoEnrich™ ATP-Binders™ Kit allows group separation of protein kinases and other ATP binding proteins, yielding partially purified cell extracts enriched in protein kinases that retain enzymatic activity. Based on an affinity resin containing immobilized ATP, the method is compatible with 2D electrophoresis, SDS-PAGE/tandem mass spectrometry, Western blot analysis, or activity assays. The unique affinity resin contains ATP covalently linked through the γ-phosphate, presenting an ideal configuration for recognition by the conserved ATP binding pocket of kinases. The kit contains the ATP-polyacrylamide-based resin, spin filters, and optimized buffers required to process extracts prepared from crude mammalian cell or tissue lysates by a standard protocol. The batchwise purification scheme is well suited for multiple separations. The kit provides sufficient reagents to perform enrichments from up to 17.5 mg crude protein.
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Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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