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EMD Chemicals, Inc.
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United States
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09 February 2012
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A set of four different monoclonal antibodies specific for serine phosphorylation sites. Recognition is dependent on phosphorylation and the surrounding amino acid motif. Recognizes serine phosphorylated proteins in Arabidopsis, bacteria, chicken, human, maize, mouse, rat, tobacco, Xenopus, yeast, and zebrafish. Phosphorylation patterns in cell extracts may differ when probed with different antibodies.
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Immunogen | phosphoserine-containing peptides or phosphoserine conjugated to KLH | ||
| Form | Lyophilized | ||
| Formulation | 25 µg each antibody lyophilized from 2X PBS, PEG, sucrose and 200 µl control phosphoproteins lyophilized 20 mM Na2HPO4, 0.1% SDS. | ||
| Preservative | ≤ 0.1% sodium azide (antibodies only) | ||
| Applications |
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| Positive control | Phosphoproteins purified from rabbit muscle (included) | ||
| Kit contains | 25 μg of each of the Phosphoserine Antibodies including clones 1C8, 4A3, 4A9, and 16B4, Rabbit Muscle Positive Control, and a data sheet. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
  Detection of phosphoserine-containing antibodies by immunoblotting. Samples: Whole cell lysate from A431 cells treated with pervanadate (all lates). Primary antibodies: PhosphoDetect™ Anti-Phosphoserine Mouse mAb (16B4) (Cat. No. 525280) (1 µg/ml), PhosphoDetect™ Anti-Phosphoserine Mouse mAb (1C8) (Cat. No. 525281) (1 µg/ml), PhosphoDetect™ Anti-Phosphoserine Mouse mAb (4A3) (Cat. No. 525283) (1 µg/ml), and PhosphoDetect™ Anti-Phosphoserine Mouse mAb (4A9) (Cat. No. 525284) (1 µg/ml). Detection: chemiluminescence.  Detection of phosphoserine-containing antibodies by immunoblotting. Samples: Extract from rabbit muscle*. Primary antibodies: PhosphoDetect™ Anti-Phosphoserine Mouse mAb (16B4) (Cat. No. 525280) (1 µg/ml), PhosphoDetect™ Anti-Phosphoserine Mouse mAb (1C8) (Cat. No. 525281) (1 µg/ml), PhosphoDetect™ Anti-Phosphoserine Mouse mAb (4A3) (Cat. No. 525283) (1 µg/ml), and PhosphoDetect™ Anti-Phosphoserine Mouse mAb (4A9) (Cat. No. 525284) (1 µg/ml). Detection: chemiluminescence. *Positive control supplied with the antibody. |
