For general questions please contact our Customer Service:
EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: Customer.CareDesk@emdchemicals.com
04 February 2012
loading
Detects and quantifies the levels of p38 MAPK protein independent of its phosphorylation state. p38 MAPK activation occurs in response to a variety of extracellular stimuli including osmotic shock, inflammatory cytokines, LPS, and UV light. Although this kit is designed for human samples, it cross-reacts with mouse and monkey.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human, monkey, mouse | ||
| Avoid freeze/thaw | Yes | ||
| Sensitivity | ≤16 pg/ml | ||
| Assay range | 31.2-2000 pg/ml | ||
| Assay time | 4 h | ||
| Sample type | Cells | ||
| Kit contains | Coated 96-Well Plate, p38 MAPK Standard, Diluents, Detector Antibody, Secondary Antibody, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Standard Handling |
||
| Data | |||
|---|---|---|---|
 The sensitivity of this ELISA was compared to immunoblotting using known quantities of p38 MAPK. The data presented in Figure 1 show that the sensitivity of the ELISA is ~10x greater than that of immunoblotting. The bands shown in the immunoblot data were developed using an anti-p38 MAPK antibody and, chemiluminescent detection. |
