For general questions please contact our Customer Service:
EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: Customer.CareDesk@emdchemicals.com
13 February 2012
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This kit uses a cell-permeable green fluorescent Cyto-dye (Ex. max.: 488 nm; Em. max.: 518 nm) to stain live cells, and propidium iodide (Ex. max.: 488 nm; Em. max.: 615 nm) to stain dead cells. Stained live and dead cells can be visualized by fluorescence microscopy using a band-pass filter which detects FITC and rhodamine. Viable cells will stain only with the Cyto-dye, fluorescing green, whereas the dead cells will stain with both Cyto-dye (green) and propidium iodide (red), resulting in a yellow fluorescence. Note: 1 T = 1 test.
Optimal staining conditions may vary among different cell types and should be determined empirically by the investigator.
| Product information | |||
|---|---|---|---|
| Format | Fluorescence microscopy | ||
| Form | 100 Tests | ||
| Detection method | Fluorescence | ||
| Species reactivity | a broad range of species | ||
| Protect from Moisture | Yes | ||
| Assay time | 0.5 h | ||
| Sample type | Cell suspensions or adherent cells | ||
| Kit contains | Staining Buffer, Cyto-Dye, Propidium Iodide, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 26-36/37/39-45 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). |
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| R Phrase |
R: 36/37/38-46 Irritating to eyes, respiratory system and skin. May cause heritable genetic damage. |
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