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70854  Introductory Mobius™ 1000 Plasmid Kit

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11 February 2012

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70854-3  2 rxn  This Product is currently discontinued or not available.
 
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The Mobius™ 1000 Plasmid Kits provide columns and buffers for isolation of up to 1 mg ultrapure plasmid DNA from 100 ml overnight bacterial culture when isolating high-copy number plasmids (Hendriks 2000). For low-copy number plasmids, the kit can isolate up to 200 µg DNA from a 250-ml culture, and up to 1 mg from 500-ml to 1.5-L cultures using a scalable protocol. The Mobius 1000 Kits incorporate a novel ClearSpin filtration device that minimizes the duration of centrifugation steps required for the clarification of bacterial lysates.
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70853 Mobius™ 1000 Plasmid Kit
Product information
Components
2Mobius 1000 Columns
2ClearSpin Filters
0.16 mlRNase A
16 mlBacterial Resuspension Buffer
16 mlBacterial Lysis Buffer
16 mlMobius Neutralization Buffer
20 mlMobius Equilibration Buffer
40 mlMobius Wash Buffer
10 mlMobius Elution Buffer
1 mlTE Buffer
Store and ship information
Storage +15°C to +30°C
Ship Shipped at Ambient Temperature or with Blue Ice
Multiple Toxicity Values, refer to MSDS

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© Merck KGaA, Darmstadt, Germany, 2012

All references to Merck refer to Merck KGaA, Darmstadt, Germany.

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