361526
GSK-3β, Rabbit Skeletal Muscle, Recombinant, E. coli
GSK 3β-Isozyme, Rabbit Skeletal Muscle, Recombinant, E. coli
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EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: Customer.CareDesk@emdchemicals.com
09 February 2012
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Recombinant, rabbit glycogen synthase kinase 3β expressed in E. coli. Dual specificity kinase. One of several protein kinases that phosphorylates glycogen synthase. Other substrates include p90rsk, Tau, c-Jun, and CREB. Plays a key role in Wnt and insulin signaling.
Note: 1 KU = 1000 units.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 50 mM NaCl, 30 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, 0.03% BRIJ®-35 Detergent, pH 7.5 | ||
| Molar mass | 47000 | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥5,000,000 units/mg protein | ||
| Unit definition | One unit is defined as the amount of GSK-3 required to catalyze the transfer of 1 pmol phosphate to CREB phosphopeptide KRREILSRRPS(P)YRP (200 µM), in 1 min at 30°C in a reaction volume of 25 µl. | ||
| Activity | ≥250,000 units/ml | ||
| Contaminants | DNase: <10% conversion of RFI DNA to RFII and linear forms. Phosphatases and Proteases: none detected. RNases: <10% RNA degradation. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
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