361523
GSK-3α, GST-Fusion, Human, Recombinant, S. frugiperda
Glycogen Synthase Kinase 3α
For general questions please contact our Customer Service:
EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: Customer.CareDesk@emdchemicals.com
11 February 2012
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Full-length, recombinant, human GSK3α fused at the N-terninus to GST and expressed in S. frugiperda insect cells using a baculovirus expression system. GSK3α is unique in its preference for a priming phosphate at n+4 (where n is the site of phosphorylation by GSK3α) in order to phosphorylate many of its substrates. This motif is found in several well-established substrates of GSK3α, such as glycogen synthase, the epsilon subunit of eIF2B, and ATP citrate-lyase. The active kinase is useful in the study of GSK3α regulation and for inhibitor screening.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 100 mM NaCl, 25 mM Tris-HCl, 10 mM reduced glutathione, 3 mM DTT, 50% glycerol, 0.05% Tween®-20 detergent, pH 8.0. | ||
| Molar mass | 81500 | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥700 units/mg protein | ||
| Unit definition | One unit is defined as the amount of enzyme that will phosphorylate 1 nmol glycogen synthase peptide substrate (YRRAAVPPSPSLSRHSSPHQSEDEEE) per min at 30°C, pH 7.4. | ||
| Purity | ≥80% by SDS-PAGE | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. |
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