341591
FGF-1, Acidic, Human, Recombinant, E. coli
rhaFGF, Fibroblast Growth Factor, Acidic, Human, Recombinant, E. coli
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EMD Chemicals, Inc.
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08027 Gibbstown
United States
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09 February 2012
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Recombinant, human acidic fibroblast growth factor expressed in E. coli. Stimulates the proliferation of primary cultures of myoblasts and osteoblastic cells. Prevents myocardial apoptosis triggered by ischemia reperfusion injury. Exhibits mitogenic activity in vitro for cells or meso- and neuroectodermal origin. Its activity is greatly potentiated by addition of heparin.
| Product information | |||
|---|---|---|---|
| Form | Solid | ||
| Formulation | Lyophilized from a sterile-filtered solution of 20 mM Tris, 1 M NaCl, 50 µg BSA/µg FGF acidic, pH 7.0. | ||
| Molar mass | 15000 | ||
| Biological activity | ED50 = 100-300 pg/ml in the presence of 10 µg/ml heparin as monitored in a mitogenic assay by measuring the acidic FGF-dependent 3H-thymidine incorporation in quiescent NR6R/3T3 fibroblasts | ||
| Purity | ≥97% by SDS-PAGE | ||
| Contaminants | endotoxin: ≤ 1 EU/µg FGF | ||
| Reconstitution | Reconstitute to a concentration of ≥1 μg/ml with sterile PBS containing at least 0.1% HSA or BSA. Reconstituted material can be stored at +4°C for up to 3 months without detectable loss of activity. For longer storage, aliquot and freeze at -70°C. Avoid freeze/thaw cycles of solutions. | ||
| Solubility | Sterile PBS containing ≥0.1% HSA or BSA | ||
| RTECS | LK3641300 | ||
| CAS number | 106096-92-8 | ||
| Merck USA index | 14, 4072 | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Ambient Temperature Only
Standard Handling |
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