For general questions please contact our Customer Service:
EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: Customer.CareDesk@emdchemicals.com
13 February 2012
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This kit provides a simple and convenient assay for measuring the activity of caspase-2 and related caspases that recognize the sequence VDVAD. The assay is based on detection of the cleavage of the substrate VDVAD-AFC. Upon cleavage of the substrate by caspase-2 or related caspases, free AFC emits a yellow-green fluorescence, which can be measured using a fluorometer or a fluorescence microplate reader (~505 nm). The kit can be used for up to 100 tests.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Fluorometric | ||
| Protect from Light | Yes | ||
| Assay time | 2.5-3 h | ||
| Sample type | Cell lysates | ||
| Kit contains | Cell Lysis Buffer, Reaction Buffer, VDVAD-AFC Substrate, DTT, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Ambient Temperature Only
Multiple Toxicity Values, refer to MSDS |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 26-36-39-61 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing. Wear eye/face protection. Avoid release to the environment. Refer to special instructions/safety data sheet. |
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| R Phrase |
R: 22-36/38-41-52/53 Harmful if swallowed. Irritating to eyes and skin. Risk of serious damage to eyes. Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment. |
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