For general questions please contact our Customer Service:
EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: Customer.CareDesk@emdchemicals.com
13 February 2012
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A convenient assay kit useful for measuring the protease activity of caspase-1 and related sequences that recognize the sequence YVAD. Cleavage of the C-terminal peptide bond of this substrate releases p-nitroaniline which absorbs at 405 nm. Assays are performed in a convenient 96-well plate format. Also useful for screening caspase-1 inhibitors.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Avoid freeze/thaw | Yes | ||
| Assay time | 2.5-3 h | ||
| Sample type | Purified protein | ||
| Kit contains | Human Recombinant Caspase-1 (Cat. No. 218783), a colorimetric caspase-1 substrate (Ac-YVAD-pNA; Cat. No. 400025), calibration standard (p-Nitroaniline; Cat. No. 483350), a caspase-1 inhibitor I (Ac-YVAD-CHO; Cat. No. 400010), assay buffer, one-half volume 96-well plate, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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