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EMD Chemicals, Inc.
480 S. Democrat Road
08027 Gibbstown
United States
Phone: 800 854 3417
Fax: 800 776 0999
E-mail: Customer.CareDesk@emdchemicals.com
04 February 2012
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Detects and quantifies the level of c-Kit protein independent of its phosphorylation state in human cells. c-Kit is a member of the receptor tyrosine kinase subfamily that includes PDGF, CSF-1, and FLT-3/flk-2 receptors. It plays a critical role in controlling a variety of cellular functions in hematopoietic stem cells, mast cells, melanocytes, and germ cells.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human, not mouse, not rat | ||
| Sensitivity | < 15 pg/ml | ||
| Assay range | 19.5 - 1250 pg/ml | ||
| Assay time | 4 h | ||
| Sample type | Cell lysate | ||
| Kit contains | c-Kit Standard, Standard Diluent Buffer, c-Kit Antibody-Coated 96-Well Plate, Rabbit Anti-c-Kit Detector Antibody, Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
||
| Canadian export regulations | Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada. | ||
| Data | |||
|---|---|---|---|
 The sensitivity of this ELISA was compared to immunoblotting using known quantities of c-Kit. The data show that the sensitivity of the ELISA is approximately 4X greater than that of immunoblotting. The bands shown in the immunoblot data were developed using a rabbit anti-c-Kit antibody and chemiluminescent detection.  Mo7e cells were treated with Stem Cell Factor (SCF) at varying concentrations (1 to 100 ng/ml) for 10 min, lysed, and quantitated in parallel for total c-Kit and c-Kit (pTyr<sup>823</sup>) expression. The amount of total c-Kit remains relatively constant, while the level of phosphorylation at Tyr<sup>823</sup> increases with the dose of SCF. The results correlate very well with immunoblot of the same samples (inset). |
