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Recognizes the ~185 kDa c-ErbB2/c-Neu protein in HEK293 cells.
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Product information
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Host
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Mouse
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Isotype
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IgG1
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Immunogen
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a synthetic peptide (TAENPEYLGLDVPV) corresponding to amino acids 1242-1255 from the C-terminal domain of human c-neu
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Clone
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3B5
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Form
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Liquid
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Formulation
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In 50 mM sodium phosphate buffer, 50% glycerol, pH 7.5.
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Species reactivity
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human, mouse
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Preservative
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None
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Applications
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Immunofluorescence
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Frozen Sections
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Immunoblotting (Western Blotting)
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Immunoprecipitation
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Paraffin Sections
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Avoid freeze/thaw
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Yes
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Positive control
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SK-BR-3 cells or breast carcinoma tissue
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Negative control
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HepG2 cells or normal skin
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Store and ship information
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Storage
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-20°C
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Ship
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Dry Ice Only
Standard Handling
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Data
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 Detection of human c-ErbB2/c-neu by immunoblotting. Sample: Whole cell lysate (56 μg) from MCF-7 cells. Primary antibody: Anti-c-ErbB2/c-Neu (Ab-3) Mouse Mab (3B5) (Cat. No. OP15) (1 μg/ml). Detection: chemiluminescence.
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