PS1012
PhosphoDetect™ Anti-VEGFR2 (pTyr¹2¹4) Rabbit pAb
Anti-KDR/Flk-1, Phospho-Specific (Tyr¹2¹4), Anti-VEGF Receptor 2 (pTyr¹2¹4) Rabbit pAb
請聯絡客服人員
台灣默克股份有限公司
內湖區堤頂大道二段89號6樓
11493 台北
台灣
電話: +886-2-21621111
傳真: +886-2-87516262
電子郵件: eShop.MTAW@merckgroup.com
13 二月 2012
請稍候
Recognizes the ~200 kDa VEGFR-2 phosphorylated at Tyr1214.
| 產品資訊 | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹2¹4 phosphorylation site of human VEGFR-2 | ||
| Form | Liquid | ||
| Formulation | In Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, pH 7.3 (± 0.1). | ||
| Species reactivity | human, mouse | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
|
||
| Avoid freeze/thaw | 是 | ||
| Positive control | NIH3T3 cells transfected with full-length, wild-type human VEGFR2 or porcine aortic endothelial (PAE) cells transfected with chimeric CSF-1/mouse VEGFR2, stimulated with CSF-1 | ||
| 儲存和運送資訊 | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Irritant |
||
| 資料 | |||
|---|---|---|---|
 Detection of mouse VEGFR2, phosphorylated at Tyr<sup>1214</sup>, by immunoblotting. Samples: Whole cell lysate from porcine aortic endothelial (PAE) cells transfected with chimeric CSF-1/mouse VEGFR2, stimulated with CSF-1 (lanes 1-4,6) or left unstimulated (lane 5). Primary antibody: PhosphoDetect™ Anti-VEGFR2 (pTyr<sup>1214</sup>) Rabbit pAb (Cat. No. PS1012) (0.5 μg/ml) pre-incubated without peptide (lanes 1,5,6), with non-phosphopeptide corresponding to the immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), or the phosphopeptide immunogen (lane 4). Detection: chemiluminescence. |
