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Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Telefon: +49 6151 72-0
Fax: +49 6151 72 2000
15 Februar 2012
wird geladen
A chemically modified trypsin that is resistant to autolysis and degradation. Trypsin specifically hydrolyzes amides, peptides and proteins at the C-terminus side of arginine and lysine residues. This mass spectrometry grade trypsin is methylated, TPCK treated, and affinity purified. It is specifically designed for digestion of proteins for sequencing and peptide fragment analysis by mass spectrometry. A trypsin to protein ratio of 1:100 to 1:20 is recommended for mass spectrometry protocols. Note: 1 SET = 5 X 20 µg of modified trypsin, and 1 ml of resuspension buffer.
| Produktinformationen | |||
|---|---|---|---|
| Form | Lyophilized | ||
| Specific activity | ≥10,000 U/mg | ||
| Unit definition | One BAEE unit is defined as the ammount of enzyme required to produce a ΔA253 of 0.001 per min in a reaction volume of 3.2 ml at pH 7.6 at 25°C. | ||
| Contaminants | Chymostrypsin Activity: <3 BTEE U/mg (one BTEE unit is defined as the amount of enzyme required to hydrolyze 1 µmol BTEE per min at 25°C, pH 7.8.) | ||
| Lager- und Versandinformationen | |||
|---|---|---|---|
| Lagerkategorie | -20°C | ||
| Ship |
Blue Ice Only
Harmful |
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| Sicherheitshinweise | |||
|---|---|---|---|
| S-Satz |
S: 22-24-26-36/37-45 |
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| R-Satz |
R: 36/37/38-42 |
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