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69022  Thrombin Cleavage Capture Kit

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64293 Darmstadt
Germany
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15 February 2012

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Product number Size Quantity Price
69022-3  1 kit 
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Prices are subject to change without notice.
The Thrombin Cleavage Capture Kit is designed for cleavage of fusion proteins followed by convenient and quantitative removal of thrombin protease. The method is based on the use of Biotinylated Thrombin for digestion and its subsequent removal with Streptavidin Agarose. The kit is suitable for use with any fusion protein that contains a thrombin recognition sequence. A Cleavage Control Protein is included in the kit to monitor performance of cleavage conditions. It is cleaved into 2 fragments, which are easily visualized by SDS-PAGE.

The Cleavage Control Protein is also available separately to monitor performance of either thrombin or enterokinase cleavage conditions. The 48 kDa control protein is cleaved into two proteolytic fragments of 35 kDa and 13 kDa, which are easily visualized by SDS-PAGE.

Product information
Avoid freeze/thaw Yes
Components
50 UBiotinylated Thrombin
5 × 1 ml10X Thrombin Cleavage Buffer
2 ml1X Thrombin Dilution/Storage Buffer
2 × 0.4 mlStreptavidin Agarose
10 µgCleavage Control Protein
pkg/10Spin Filters, 2 ml capacity
Store and ship information
Storage Multiple storage requirements
Ship Blue Ice Only
Multiple Toxicity Values, refer to MSDS
Safety information
S Phrase S: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
R Phrase R: 36/37/38

Irritating to eyes, respiratory system and skin.

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