71540
Tag•off™ rEK Cleavage/Capture Kit
Increased cleavage rate of fusion proteins
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
15 February 2012
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Tag•off™ rEK Cleavage Capture Kit is designed for highly specific cleavage of fusion proteins, followed by rapid affinity-based capture and removal of enterokinase. Following cleavage of the target protein, greater than 99% of Tag•off rEK can be quickly removed from the reaction by affinity capture on EKapture Agarose available in the Tag•off rEK Cleavage Capture Kit. Just add EKapture Agarose to the rEK digest, incubate at room temperature for 5 minutes and remove the resin by spin filtration. Because the same buffer conditions are used for both cleavage and capture, no buffer change is necessary. The Tag•off rEK Cleavage Capture Kit includes the EKapture Agarose and Spin Filters in addition to the same components available with the Tag•off High Activity rEK (enzyme, dilution buffer, cleavage buffer and control protein).
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Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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