71537
Tag•off™ High Activity rEK
Tag-Off High Activity Enterokinase
Increased cleavage rate of fusion proteins
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
15 February 2012
loading
Tag•off™ High Activity rEK is a highly
purified preparation of the catalytic subunit
of recombinant human enterokinase that
recognizes the identical cleavage site as
the native enzyme, AspAspAspAspLys↓
(Gasparian 2003). The Tag•off High Activity
rEK substrate binding site (ArgArgArgLys)
differs from the bovine enterokinase site
(LysArgArgLys) by a single amino acid, which
is believed to account for its higher substrate
affinity and the resulting higher specific
activity. The preparation has been functionally
tested for activity with thioredoxin/human
epidermal growth factor and thioredoxin/
human IL-13 fusion proteins (Gasparian). The
Tag•off High Activity rEK as a highly purified
(99% by Coomassie staining) enzyme solution
and includes rEK 1X Dilution/Storage Buffer,
10X Cleavage Buffer, and a Cleavage Control
Protein.
| Product information | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Unit definition | One unit is defined as the amount of enzyme needed to cleave 50 µg of fusion protein in 16 hours at 23°C in a buffer containing 20 mM Tris-HCl, 50 mM NaCl, and 2 mM CaCl2, pH 7.4. | ||||||||||||||
| Components |
|
||||||||||||||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Shipped with Blue Ice or with Dry Ice
Multiple Toxicity Values, refer to MSDS |
||
