For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
15 February 2012
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T4 DNA Ligase catalyzes the formation of
phosphodiester bonds between 3′-hydroxyl
and 5′-phosphate groups of adjacent DNA and
RNA nucleotides in blunt-end or cohesive-end
configurations. The enzyme joins RNA
and DNA strands only in duplex molecules
and does not join single-stranded nucleic
acids. Novagen T4 DNA Ligase is rigorously
tested in a blue-white cloning assay under
conditions that maximize exposure of DNA
ends to any contaminating nucleases. The
enzyme is qualified for any ligation or cloning
application.
| Product information | |||
|---|---|---|---|
| Avoid freeze/thaw | Yes | ||
| Unit definition | 4 Weiss units/µl; one Weiss unit is defined as the amount of enzyme required to catalyze the exchange of 1 nmole of 32P from pyrophosphate into ATP as Norit®-absorbable material in 20 minutes at 37°C. 0.01 Weiss unit is the amount of enzyme required to ligate > 95% of 1 µg bacteriophage λ Hind III fragments at 16°C in 20 minutes. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Shipped with Blue Ice or with Dry Ice
Standard Handling |
||
