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71350  Rosetta-gami™ 2 Competent Cells

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15 February 2012

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Product number Size Quantity Price
71350-3  0.4 ml 
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71350-4  1 ml 
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Prices are subject to change without notice.
Rosetta-gami 2 host strains combine the advantages of Rosetta 2 and Origami 2 strains to alleviate codon bias and enhance disulfide bond formation in the cytoplasm when heterologoous proteins are expressed in E. coli. These trxB/gor mutants are compatible with kanamycin-resistant vectors, and carry the chloramphenicol-resistant pRARE2 plasmid, which supplies seven rare tRNAs. Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac+ lacIq pro] gor522::Tn10 trxB pRARE2 (CamR, StrR, TetR)
Product information
Components
0.4 ml1 mlComponent
2 × 0.2 ml5 × 0.2 mlRosetta-gami 2 Competent Cells
2 × 2 ml4 × 2 mlSOC Medium
10 µl10 µlTest Plasmid
Guaranteed efficiency 2 × 106 cfu/µg
Store and ship information
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS

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