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Rosetta-gami 2 host strains combine the advantages of Rosetta 2 and Origami 2 strains to alleviate codon bias and enhance disulfide bond formation in the cytoplasm when heterologoous proteins are expressed in E. coli. These trxB/gor mutants are compatible with kanamycin-resistant vectors, and carry the chloramphenicol-resistant pRARE2 plasmid, which supplies seven rare tRNAs.
Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac+ lacIq pro] gor522::Tn10 trxB pRARE2 (CamR, StrR, TetR)
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Product information
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Components
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| 0.4 ml | 1 ml | Component
| | • | 2 × 0.2 ml | 5 × 0.2 ml | Rosetta-gami 2 Competent Cells
| | • | 2 × 2 ml | 4 × 2 ml | SOC Medium
| | • | 10 µl | 10 µl | Test Plasmid |
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Guaranteed efficiency
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2 × 106 cfu/µg
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Store and ship information
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Storage
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≤ -70°C
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Ship
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Dry Ice Only
Multiple Toxicity Values, refer to MSDS
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