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Merck KGaA
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15 February 2012
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Assay measures the increased activity of cellular mitochondrial dehydrogenases that can cleave the tetrazolium dye WST-1 to formazan. The formazan formation is then quantified by measuring the change in absorbance at 450 nm in a microplate reader. The activity of mitochondrial dehydrogenases is proportional to cell number. No washing, harvesting, or solubilization steps are required.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 500 Tests | ||
| Detection method | Colorimetric | ||
| Protect from Light | Yes | ||
| Avoid freeze/thaw | Yes | ||
| Sensitivity | 1000 cells (adherent); 2500 cells (nonadherent) | ||
| Assay range | 1000 - 50,000 Cells | ||
| Assay time | 0.5 - 4 h | ||
| Sample type | Cultured cells | ||
| Kit contains | WST-1, 1-Methoxy PMS, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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