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The pTriEx™-7 vector is uniquely designed to allow rapid characterization of target genes in multiple expression systems. With this vector, a single recombinant plasmid can be used to test expression in E. coli, insect and mammalian cells. Transient mammalian expression is mediated by the CMV immediate early enhancer and promoter. For expression in insect cells, pTriEx-7 contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector™ System or BacMagic™ Kits. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. For expression in E. coli, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows IPTG based induction. The pTriEx-7 contains the coding sequence for the mouse IgM signal sequence to allow secretion of the expressed protein. pTriEx-7 also encodes an N-terminal 8 aa Strep•Tag ® II coding sequence followed by an enterokinase protease cleavage site and a multiple cloning site, with the option to include a C-terminal His•Tag ® coding sequence with 10 histidines. Additionally, the pTriEx-7 vector is available as an Ek/LIC Vector Kit.
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| Declaration | This product is covered by U.S. Patent 6,589,783 owned by EMD Chemicals Inc. or its Affiliates. | ||
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| Lagerkategorie | -20°C | ||
| Ship |
Shipped with Blue Ice or with Dry Ice
Standard Handling |
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