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70905  pTriEx™-4 Ek/LIC Vector Kit

InsectDirect™, pTriEx™-4 Ek/LIC Vector Kit

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15 February 2012

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Product number Size Quantity Price
70905-3  20 rxn 
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Prices are subject to change without notice.
The pTriEx™-4 Ek/LIC vector is an Ek/LIC version of pTriEx-4. The pTriEx-4 Ek/LIC vector is uniquely designed to allow rapid characterization of target genes in multiple expression systems. With this vector, a single recombinant plasmid can be used to test expression in E. coli, insect and mammalian cells. Transient mammalian expression is mediated by the CMV immediate early enhancer and promoter. For expression in insect cells, the pTriEx-4 Ek/LIC vector contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector™ System or BacMagic™ Kits. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. The pTriEx-4 Ek/LIC vector contains N-terminal His•Tag® and S•Tag™ coding sequences followed by an enterokinase protease cleavage site and a multiple cloning site, with the option to include a C-terminal His•Tag coding sequence.

This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
Product information
Components
1 µgpTriEx-4 Ek/LIC Vector
10 µlEk/LIC Control Insert
25 UT4 DNA Polymerase, LIC-qualified
50 µl10X T4 DNA Polymerase Buffer
100 µl100 mM DTT
50 µl25 mM EDTA
40 µl25 mM dATP
1.5 mlNuclease-free Water
22 × 50 µlNovaBlue GigaSingles™ Competent Cells
0.2 mlOrigami™ B(DE3)pLacI Competent Cells
5 × 2 mlSOC Medium
10 µlTest Plasmid
Declaration This product is covered by U.S. Patent 6,589,783 owned by EMD Chemicals Inc. or its Affiliates.
Store and ship information
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS
Licence Brookhaven This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

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