For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
15 February 2012
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The pT7Blue vector features the pUC19 backbone (including high-copy number origin of replication and lac sequences), a T7 promoter, f1 origin of replication, and modified multiple cloning region. The multiple cloning region contains an EcoR V site used for T-cloning flanked by an Nde I site, which allows inserts to be conveniently subcloned into the Nde I site of many pET vectors.
| Related products | |
|---|---|
| 70183 | Introductory pT7Blue Perfectly Blunt® Cloning Kit |
| 70189 | pT7Blue Perfectly Blunt® Cloning Kit |
| Related products for pT7Blue Blunt Vector (linearized) | |
| 70183 | Introductory pT7Blue Perfectly Blunt® Cloning Kit |
| 70189 | pT7Blue Perfectly Blunt® Cloning Kit |
| Product information | ||||||||||||||||||
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| Avoid freeze/thaw | Yes | |||||||||||||||||
| Components |
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| Features and benefits |
* All times listed refer to kits using NovaBlue Singles™ Competent Cells and ampicillin or chloramphenicol selection. Giga kits, which use NovaBlue GigaSingles™ Competent Cells, require one hour for outgrowth. |
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| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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