The pT7Blue vector features the pUC19 backbone (including high-copy number origin of replication and lac sequences), a T7 promoter, f1 origin of replication, and modified multiple cloning region. The multiple cloning region contains an EcoR V site used for T-cloning flanked by an Nde I site, which allows inserts to be conveniently subcloned into the Nde I site of many pET vectors.
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Produktinformationen
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Avoid freeze/thaw
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Ja
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Components
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| 20 rxn | 40 rxn
| | • | 2 × 0.5 µg | 4 × 0.5 µg | Blunt Vector
| | • | 10 µl | 10 µl | Positive Control Insert
| | • | 100 µl | 2 × 100 µl | End Conversion Mix |
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Features and benefits
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- No restriction enzymes or special primers
- Compatible with any DNA polymerase
- Independent of 3′-dA addition or sequence context of DNA ends
- Blue/white screening with seven different vectors, including pETBlue expression vectors
- Simple protocol takes less than one hour* from PCR product to plating transformants
* All times listed refer to kits using NovaBlue Singles™ Competent Cells and ampicillin or chloramphenicol selection. Giga kits, which use NovaBlue GigaSingles™ Competent Cells, require one hour for outgrowth.
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Lager- und Versandinformationen
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Lagerkategorie
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≤ -70°C
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Ship
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Dry Ice Only
Standard Handling
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