71228
pSTBlue-1 AccepTor™ Vector Giga Kit
Featuring super-efficient competent cells for increased transformation efficiency
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
15 February 2012
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The pSTBlue-1 AccepTor™ Vector Giga Kits and pSTBlue-1 Perfectly Blunt® Giga Cloning Kits feature NovaBlue GigaSingles™ Competent Cells for increased transformation efficiency. AccepTor Vector Kits are designed for the cloning of PCR products generated using thermostable DNA polymerases that leave single 3′-dA nucleotide overhangs. The vector enables direct ligation by virtue of single 3′-dU overhangs that anneal with 3′-dA extensions on PCR products. The dU residues are converted to dT residues in vivo.
The Perfectly Blunt Cloning Kits are designed for the cloning of DNA generated by PCR using any type of DNA polymerase. This allows for the use of high-fidelity proofreading enzymes for amplification, thereby decreasing the probability of generating mutations in the target sequence.
| Related products for pSTBlue-1 AccepTor™ Vector Giga Kit | |
| 70594 | Introductory pSTBlue-1 AccepTor™ Vector Kit |
| 70595 | pSTBlue-1 AccepTor™ Vector Kit |
| 70596 | pSTBlue-1 AccepTor™ Vector (linearized vector) |
| 70597 | Introductory pETBlue-1 AccepTor™ Vector Kit |
| 70598 | pETBlue-1 AccepTor™ Vector Kit |
| 70599 | pETBlue-1 AccepTor™ Vector (linearized vector) |
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| Components |
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| Features and benefits |
• No restriction enzymes or special primers • Direct ligation of PCR product with vector • Compatible with polymerases that leave single 3´-dA overhangs • Blue/white screening with pSTBlue-1 or pETBlue™-1 vectors • Simple protocol takes as little as 40 minutes from PCR product to plating transformants |
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| Declaration | This product is covered by U.S. Patent 5,856,144 owned by EMD Chemicals Inc. or its Affiliates. | |||||||||||||||||||||||
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| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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