539789
ProteoExtract® Partial Mammalian Proteome Extraction Kit
P-PEK Mammalian Kit
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
15 February 2012
loading
This product has been discontinued.
We are currently only offering the ProteoExtract® Complete Mammalian Proteome Extraction Kit (Cat. No. 539779) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.
The Partial Mammalian Proteome Extraction Kit (P-PEK, Mammalian) is designed for serial sample preparation of complex mixtures using reagents with increasing solubilization strength. Each of the provided extraction reagents solubilizes a different subset of cell proteins. The most soluble of the mammalian proteins are released by mechanical destruction of the cells in Extraction Reagent 1, proteins of intermediate solubility are subsequently extracted with reagent 2, then the membrane proteins can be extracted using Extraction Reagent 3. These fractions can then be analyzed on separate 2-D gel electrophoresis and the insoluble fraction can be analyzed by one-dimensional SDS-PAGE using the SDS buffers provided. To preserve the protein profile and increase spot resolution, the kit also contains Protease Inhibitor Cocktail and the proprietary non-specific nuclease, Benzonase® Nuclease (Cat. No. 71206). Sample size: 1-2x108 cells or 500 mg tissue.
| Product information | |||
|---|---|---|---|
| Form | 20 Extractions | ||
| Avoid freeze/thaw | Yes | ||
| Sample type | Mammalian cells | ||
| Kit contains | Wash Buffer, Extraction Reagent 1, Extraction Reagent 2, Extraction Reagent 3A, Extraction Reagent 3B, SDS Buffer A, SDS Buffer B, Glass Beads, Protease Inhibitor Cocktail, Benzonase Nuclease (Cat. No. 71206), and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Ambient Temperature Only
Multiple Toxicity Values, refer to MSDS |
||
