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14 febrero 2012
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The pLacI plasmid is a chloramphenicol resistant plasmid that encodes the lac repressor protein. The p15a origin of replication is compatible with colE1 based vectors, including Novagen's pETBlue™ and pTriEx™ plasmids. These expression vectors contain lac operators to regulate T7 promoter-driven expression, but do not supply the lac repressor. Thus co-transformation with pLacI supplies a source of lac repressor protein to maintain repression of these vectors in λDE3 lysogenic bacterial expression host strains. Expression of lacI is driven by a constitutive E. coli promoter. Unique restriction sites are indicated on the vector map (TB269).
| Productos relacionados | |
|---|---|
| 69418 | pET-3a DNA |
| 69419 | pET-3b DNA |
| 69420 | pET-3c DNA |
| 69421 | pET-3d DNA |
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