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15 February 2012
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Detects and quantifies the level of c-Met phosphorylated at Tyr1230/1234/1235. c-Met is a proto-oncogene and a cell-surface receptor for hepatocyte growth factor, which plays an important role in signal transduction process. It is overexpressed in many cancers and often amplified between tumorigenesis and metastasis, so it could be a useful prognostic marker.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human | ||
| Unit definition | One unit is defined as the amount of c-Met (pTyr1230/1234/1235) derived from 21,000 GTL 16 cells treated with 1 mM sodium orthovanadate for 16 h. | ||
| Sensitivity | <0.25 Units/ml | ||
| Assay range | 1.56-100 Units/ml | ||
| Assay time | 4 h | ||
| Sample type | Cells | ||
| Positive control | A549 cells | ||
| Kit contains | c-Met (pTyr1230/1234/1235) Standard, Standard Diluent Buffer, c-Met Antibody-Coated 96-Well Plate, Rabbit Anti-c-Met (pTyr1230/1234/1235) Detector Antibody, Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Data | |||
|---|---|---|---|
 The sensitivity of this ELISA was compared to immunoblotting using known quantities of c-Met (pTyr<sup>1230/1234/1235</sup>). The data show that the sensitivity of the ELISA is approximately 2x greater than that of immunoblotting. The bands shown in the immunoblot data were developed using an anti-c-Met (pTyr<sup>1230/1234/1235</sup>) antibody and chemiluminescent detection. |
