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Merck4Biosciences

WideScreen®

Calbiochem

Novabiochem

Novagen

69307  PhageMaker® System

High-efficiency one-tube λ packaging

For general questions please contact our Customer Service:

Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000

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15 February 2012

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Product number Size Quantity Price
69307-3  6 ea  This Product is currently discontinued or not available.
69307-4  11 ea  This Product is currently discontinued or not available.
Prices are subject to change without notice.
The Novagen PhageMaker System is optimized for maximum packaging efficiencies of cDNA and genomic DNA inserts ligated with any bacteriophage λ vector. This one-tube system is extremely easy to use: simply add up to 5 µg of ligated vector plus insert to the extract and incubate at room temperature for 2 hours.
Product information
Avoid freeze/thaw Yes
Components
11 × 50 µlPhageMaker Extracts
2 µgPhageMaker Control DNA
0.2 mlLE392 Host Strain
Features and benefits
  • Packaging efficiency of > 2 × 108 pfu/µg DNA
  • The negligible background of < 10 pfu per extract is orders of magnitude lower than conventional two-extract systems.
  • The extract is devoid of EcoK and other restriction systems (mcrA, mcrBC, mrr) that recognize methylated DNA. Therefore, the PhageMaker® system is especially recommended for constructing highly representative cDNA and genomic DNA libraries in which the insert DNA is methylated.
  • The one-tube configuration enables the use of multiple aliquots from the same tube, thus providing a significant cost savings over other systems.
Store and ship information
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS

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