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The Novagen PhageMaker System is optimized
for maximum packaging efficiencies of cDNA
and genomic DNA inserts ligated with any
bacteriophage λ vector. This one-tube system
is extremely easy to use: simply add up to 5
µg of ligated vector plus insert to the extract
and incubate at room temperature for 2 hours.
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Product information
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Avoid freeze/thaw
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Yes
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Components
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| • | 11 × 50 µl | PhageMaker Extracts
| | • | 2 µg | PhageMaker Control DNA
| | • | 0.2 ml | LE392 Host Strain |
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Features and benefits
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- Packaging efficiency of > 2 × 108 pfu/µg DNA
- The negligible background of < 10 pfu per extract is orders of magnitude lower than conventional two-extract systems.
- The extract is devoid of EcoK and other restriction systems (mcrA, mcrBC, mrr) that recognize methylated DNA. Therefore, the PhageMaker® system is especially recommended for constructing highly representative cDNA and genomic DNA libraries in which the insert DNA is methylated.
- The one-tube configuration enables the use of multiple aliquots from the same tube, thus providing a significant cost savings over other systems.
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Store and ship information
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Storage
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≤ -70°C
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Ship
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Dry Ice Only
Multiple Toxicity Values, refer to MSDS
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