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Merck KGaA
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Phone: +49 6151 72-0
Fax: +49 6151 72 2000
15 February 2012
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pETDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS), each of which is preceded by a T7 promoter, lac operator and ribosome binding site (rbs). The vector also carries the pBR322-derived ColE1 replicon, lacI gene and ampicillin resistance gene. This vector can be transformed into the same cell with pACYCDuet™-1, pCDFDuet™-1, and pRSFDuet™-1 or pCOLADuet™-1 in the appropriate host strain for the coexpression of up to eight target genes. Genes inserted into MCS-1 can be sequenced using the pETUpstream Primer and DuetDOWN1 Primer. Genes inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.
| Product information | |||
|---|---|---|---|
| Fusion tag | His•Tag, S•Tag | ||
| Vector family | Duet pET bacterial prokaryotic | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Shipped with Blue Ice or with Dry Ice
Standard Handling |
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| Licence Brookhaven | This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license. | ||
