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70621  pETBlue™-2 Blunt Vector (linearized)

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Merck KGaA
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64293 Darmstadt
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Phone: +49 6151 72-0
Fax: +49 6151 72 2000

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15 February 2012

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Product number Size Quantity Price
70621-3  20 rxn 
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70621-4  40 rxn 
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Prices are subject to change without notice.
The pETBlue™-2 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes. Screening is independent of expression because the T7lac expression promoter is in an opposed orientation relative to the E. coli promoter that mediates blue/white screening. pETBlue-2 defines the open reading frame and inserts must be cloned in-frame if expression is desired. The vector features an expanded multiple cloning site (MCS) and optional C-terminal HSV•Tag® and His•Tag® sequences. The sequence is numbered from the first base of the T7 promoter sequence.


In addition to the pETBlue™ Systems, which contain uncut plasmids, pETBlue vectors are available in formats ready for insertion of PCR products. Two types of kits are available:
AccepTor™ Vector Kits and Perfectly Blunt® Cloning Kits. The AccepTor Vector Kit enables direct insertion of DNA containing single 3′-dA overhangs, such as those amplified with non-proofreading DNA polymerases (e.g., NovaTaq™ DNA Polymerase), while the Perfectly Blunt Cloning Kits are designed for cloning inserts having any type of DNA end.


Product information
Avoid freeze/thaw Yes
Components
20 rxn40 rxn
•2 × 0.5 µg4 × 0.5 µgBlunt Vector
•10 µl10 µlPositive Control Insert
•100 µl2 × 100 µlEnd Conversion Mix
Features and benefits
  • No restriction enzymes or special primers
  • Compatible with any DNA polymerase
  • Independent of 3′-dA addition or sequence context of DNA ends
  • Blue/white screening with seven different vectors, including pETBlue expression vectors
  • Simple protocol takes less than one hour* from PCR product to plating transformants

* All times listed refer to kits using NovaBlue Singles™ Competent Cells and ampicillin or chloramphenicol selection. Giga kits, which use NovaBlue GigaSingles™ Competent Cells, require one hour for outgrowth.
Store and ship information
Storage ≤ -70°C
Ship Dry Ice Only
Standard Handling

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