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pET Expression Systems and pET
pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.
Components: pET Expression Systems
Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
• 10 µg pET vector DNA (for each indicated plasmid)
• 0.2 ml BL21 Glycerol Stock
• 0.2 ml BL21(DE3) Glycerol Stock
• 0.2 ml BL21(DE3)pLysS Glycerol Stock
• 0.2 ml Induction Control Glycerol Stock
Components: pET Expression Systems plus Competent Cells
pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
• 0.2 ml NovaBlue Competent Cells
• 0.2 ml BL21(DE3) Competent Cells
• 0.2 ml BL21(DE3)pLysS Competent Cells
• 2 × 0.2 ml SOC Medium
• 10 µl Test Plasmid
These components are sufficient for up to 10 transformations in each host.
Purification and Detection Reagents
Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.
pET Expression System 17b plus Competent Cells
The pET-3, 9, and 11 vector series are original, basic pET plasmids constructed by Studier and colleagues (Studier 1990) and are the precursors of the subsequent pET vectors. These vectors offer a single BamH I cloning site in three reading frames for producing proteins fused with a non-cleavable Nterminal T7•Tag® epitope. Unfused proteins can be produced by using the Nde I cloning site in the "a", "b", and "c" versions, or the Nco I site in the "d" version. The pET-17b vector carries a multiple cloning site in oneframe.
This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.
Components: pET Expression Systems
Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
• 10 µg pET vector DNA (for each indicated plasmid)
• 0.2 ml BL21 Glycerol Stock
• 0.2 ml BL21(DE3) Glycerol Stock
• 0.2 ml BL21(DE3)pLysS Glycerol Stock
• 0.2 ml Induction Control Glycerol Stock
Components: pET Expression Systems plus Competent Cells
pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
• 0.2 ml NovaBlue Competent Cells
• 0.2 ml BL21(DE3) Competent Cells
• 0.2 ml BL21(DE3)pLysS Competent Cells
• 2 × 0.2 ml SOC Medium
• 10 µl Test Plasmid
These components are sufficient for up to 10 transformations in each host.
Purification and Detection Reagents
Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.
pET Expression System 17b plus Competent Cells
The pET-3, 9, and 11 vector series are original, basic pET plasmids constructed by Studier and colleagues (Studier 1990) and are the precursors of the subsequent pET vectors. These vectors offer a single BamH I cloning site in three reading frames for producing proteins fused with a non-cleavable Nterminal T7•Tag® epitope. Unfused proteins can be produced by using the Nde I cloning site in the "a", "b", and "c" versions, or the Nco I site in the "d" version. The pET-17b vector carries a multiple cloning site in oneframe.
This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
| Related products | |
|---|---|
| 69405 | pET Expression System 11 |
| 69406 | pET Expression System 11 plus Competent Cells |
| 69409 | pET Expression System 3 |
| 69410 | pET Expression System 3 plus Competent Cells |
| Product information | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Avoid freeze/thaw | Yes | ||||||||||||||||||||
| Components |
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| Fusion tag | T7•Tag | ||||||||||||||||||||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Licence Brookhaven | This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license. | ||
