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Merck4Biosciences

WideScreen®

Calbiochem

Novabiochem

Novagen

70789  pET Dsb Fusion System 39b

Vectors for export and periplasmic folding of target proteins

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15 febrero 2012

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Número de producto Tamaño Cantidad Precio
70789-3  1 ea 
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Los precios están sujetos a cambios sin notificación.

The pET Dsb Fusion Systems 39b and 40b are designed for cloning and high-level expression of peptide sequences fused with the 208-aa DsbA•Tag™ sequence [pET-39b(+)] or the 236-aa DsbC•Tag™ sequence [pET-40b(+)].

DsbA and DsbC are periplasmic enzymes that catalyze the formation and isomerization of disulfide bonds, respectively (Rietsch 1996, Sone 1997, Missiakas 1994, Zapun 195, Raina 1997). In contrast to the pET-32 Trx•Tag™ series, which is designed to increase solubility in the cytoplasm, the DsbA•Tag and DsbC•Tag vectors enable potential periplasmic localization and thus may enhance solubility and proper folding in this non-reducing environment (Collins-Racie 1995).





This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
Información sobre producto
Avoid freeze/thaw Si
Components
•10 µgpET vector DNA
•0.2 mlHost bacterial strains BL21, BL21(DE3), and BL21(DE3)pLysS, glycerol stocks
•0.2 mlInduction control clone, glycerol stock
•1Novagen Vector CD containing all vector sequences and maps
Fusion tag His•Tag, S•Tag
Almacenar y enviar información
Categoría de alamacenamiento ≤ -70°C
Ship Shipped with Blue Ice or with Dry Ice
Standard Handling
Licence Brookhaven This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

© Merck KGaA, Darmstadt, Alemania, 2012

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