The pET-42 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (
TB240). The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Vector encoded sequence can be completely removed when cloning into the
PshAI site (as shown below) and then cleaving the GST fusion protein with Factor Xa...
This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
|
Product information
|
|
Avoid freeze/thaw
|
Yes
|
|
Store and ship information
|
|
|
|
Storage
|
≤ -70°C
|
|
Ship
|
Shipped with Blue Ice or with Dry Ice
Standard Handling
|
|
Licence Brookhaven
|
This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
|
