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Directional PCR cloning into the most powerful E. coli expression vectors
The pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. Vector encoded sequence can be completely removed by cleaving the GST fusion protein with enterokinase. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB317). The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
The pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. Vector encoded sequence can be completely removed by cleaving the GST fusion protein with enterokinase. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB317). The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
| Related products | |
|---|---|
| 69076 | pET-32 Ek/LIC Vector Kit |
| 69077 | pET-30 Ek/LIC Vector Kit |
| 70072 | pET-32 Xa/LIC Vector Kit |
| 70073 | pET-30 Xa/LIC Vector Kit |
| Related products for pET-41 Ek/LIC Vector Kit | |
| 69076 | pET-32 Ek/LIC Vector Kit |
| 69077 | pET-30 Ek/LIC Vector Kit |
| 70072 | pET-32 Xa/LIC Vector Kit |
| 70073 | pET-30 Xa/LIC Vector Kit |
| 71072 | pET-43.1 Ek/LIC Vector Kit |
| 71144 | pET-44 Ek/LIC Vector Kit |
| 71335 | pET-46 Ek/LIC Vector Kit |
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| Vector family | pET | |||||||||||||||||||||||||||||||||||||||||
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| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Licence Brookhaven | This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license. | ||
