70091
pET-40b(+) DNA
Vectors for export and periplasmic folding of target proteins
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
15 February 2012
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The pET Dsb Fusion Systems 39b and 40b are designed for cloning and high-level expression of peptide sequences fused with the 208-aa DsbA•Tag™ sequence [pET-39b(+)] or the 236-aa DsbC•Tag™ sequence [pET-40b(+)].
DsbA and DsbC are periplasmic enzymes that catalyze the formation and isomerization of disulfide bonds, respectively (Rietsch 1996, Sone 1997, Missiakas 1994, Zapun 195, Raina 1997). In contrast to the pET-32 Trx•Tag™ series, which is designed to increase solubility in the cytoplasm, the DsbA•Tag and DsbC•Tag vectors enable potential periplasmic localization and thus may enhance solubility and proper folding in this non-reducing environment (Collins-Racie 1995).
The pET-40b(+) vector is designed for expression of DsbC fusion proteins. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB229). The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand.
The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.
This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
| Related products | |
|---|---|
| 70090 | pET-39b(+) DNA |
| 70789 | pET Dsb Fusion System 39b |
| 70790 | pET Dsb Fusion System 39b plus Competent Cells |
| 70791 | pET Dsb Fusion System 40b |
| Product information | |||
|---|---|---|---|
| Avoid freeze/thaw | Yes | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Shipped with Blue Ice or with Dry Ice
Standard Handling |
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| Licence Brookhaven | This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license. | ||
