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Novagen

70072  pET-32 Xa/LIC Vector Kit

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15 February 2012

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Product number Size Quantity Price
70072-3  20 rxn 
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Prices are subject to change without notice.
The pET-32 Xa/LIC vector is an Xa/LIC version of pET-32b(+). It is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag™ thioredoxin protein, and His•Tag® and S®Tag™ coding sequences that are cleavable with Factor Xa protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Xa protease cleavage site (IleGluGlyArg↓), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal His•Tag coding sequence is compatible with purification, detection, and quantification...

This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
Product information
Avoid freeze/thaw Yes
Components
1 µgXa/LIC Vector
10 µlXa/LIC Control Insert
25 UT4 DNA Polymerase, LIC-qualified
50 µl10X T4 DNA Polymerase Buffer
100 µl100 mM DTT
50 µl25 mM EDTA
40 µl25 mM dGTP
1.5 mlNuclease-free Water
22 × 50 µlNovaBlue GigaSingles™ Competent Cells
0.2 mlBL21(DE3) Competent Cells
0.2 mlBL21(DE3)pLysS Competent Cells
5 × 2 mlSOC Medium
10 µlTest Plasmid
Store and ship information
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS
Licence Brookhaven This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

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