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71337  pCDF-2 Ek/LIC Vector Kit

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15 February 2012

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Product number Size Quantity Price
71337-3  20 rxn 
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Prices are subject to change without notice.
The pCDF-2 Ek/LIC Vector is a LIC-compatible derivative of pCDF-1b. The plasmid contains a strong T7lac promoter and an amino terminal His•Tag® coding sequence immediately followed by an Ek/LIC cloning site designed to allow the generation of fusion proteins with minimal vector-encoded sequence. An optional C-terminal S•Tag™ coding sequence is compatible with purification, detection, and quantification (1). This plasmid is compatible with the LIC Duet Adaptors for coexpression of two proteins as well as with pET-46 and pRSF-2 Ek/LIC vectors for coexpression of up to six proteins when using the LIC Duet Adaptors.

This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
Product information
Components
1 µgEk/LIC Vector
25 unitsT4 DNA Polymerase, LIC-qualified
50 µl25 mM EDTA
40 µl25 mM dATP
8 µlEk/LIC β-gal Control Insert
100 µl100 mM DTT
50 µl10X T4 DNA Polymerase Buffer
1.5 mlNuclease-free Water
5 × 2 mlSOC Medium
10 µlTest Plasmid
0.2 mlBL21(DE3)pLysS Competent Cells
0.2 mlBL21(DE3) Competent Cells
22 × 50 µlNovaBlue GigaSingles™ Competent Cells
Store and ship information
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS
Licence Brookhaven This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

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