The pBAC-2cp Ek/LIC vector is an Ek/LIC version of pBAC-2cp. The pBAC-2cp Ek/LIC vector is a baculovirus transfer plasmid designed for simplified cloning and expression of target genes in insect cells. The plasmid is compatible with BacVector™ Triple Cut Virus DNA and BacMagic™ DNA for low background transfection and efficient utilization of the polh promoter. pBAC-2cp Ek/LIC vector provides an ATG start codon at the optimal position relative to native polyhedrin translation signals. Cloning sites are provided for the creation of N-terminal fusions of an insert with His•Tag® and S•Tag™ sequences followed by an enterokinase protease cleavage site and a multiple cloning site, with the option to include a C-terminal His•Tag coding sequence.
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Product information
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Avoid freeze/thaw
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Yes
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Components
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| • | 1 µg | pBAC-2cp Ek/LIC Transfer Plasmid
| | • | 10 µl | LIC gus Control Insert
| | • | 25 U | T4 DNA Polymerase, LIC-qualified
| | • | 50 µl | 10X T4 DNA Polymerase Buffer
| | • | 100 µl | 100 mM DTT
| | • | 25 µl | 25 mM dATP
| | • | 50 µl | 25 mM EDTA
| | • | 1.5 ml | Nuclease-free Water
| | • | 22 × 50 µl | NovaBlue GigaSingles™ Competent Cells
| | • | 4 × 0.2 ml | SOC Medium
| | • | 10 µl | Test Plasmid |
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Store and ship information
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Storage
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≤ -70°C
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Ship
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Dry Ice Only
Multiple Toxicity Values, refer to MSDS
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