For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
15 February 2012
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pBAC™-1 is a baculovirus transfer plasmid designed for simplified cloning and expression of target genes in insect cells. The plasmid is compatible with BacVector™-1000, -2000 or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the polh promoter. Inserts in pBAC-1 must contain their own translation initiation codon. The vector encodes an optional C-terminal His•Tag® fusion sequence that may be utilized if the insert allows read-through in the proper reading frame. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Single stranded sequencing of phage-derived DNA can be performed using the 1629DWN primer.
| Related products | |
|---|---|
| 70004 | pBAC™-2cp DNA |
| 70049 | pBACgus-2cp DNA |
| 70054 | pBAC™gus-1 DNA |
| 70088 | pBAC™-3 DNA |
| Related products for pBAC™-1 DNA | |
| 70004 | pBAC™-2cp DNA |
| 70049 | pBACgus-2cp DNA |
| 70054 | pBAC™gus-1 DNA |
| 70088 | pBAC™-3 DNA |
| 70089 | pBACgus-3 DNA |
| Product information | |||
|---|---|---|---|
| Avoid freeze/thaw | Yes | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Shipped with Blue Ice or with Dry Ice
Standard Handling |
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