For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
14 February 2012
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This product has been discontinued.
We are offering Origami™ 2 Competent Cells (Cat. No. 71344) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.
Origami™ host strains are K-12 derivatives that have mutations in both the thioredoxin reductase (trxB) and glutathione reductase (gor) genes, which together greatly enhance disulfide bond formation in the cytoplasm. Studies have shown that expression in Origami(DE3) yielded 10-fold more active protein than in another host even though overall expression levels were similar (1). Origami hosts are compatible with ampicillin-resistant plasmids and are ideal for use with pET-32 vectors, since the thioredoxin fusion tag further enhances the formation of disulfide bonds in the cytoplasm. Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac+ lacIq pro] gor522::Tn10 trxB (KanR, StrR, TetR)
| Related products | |
|---|---|
| 70627 | Origami™(DE3) Competent Cells |
| Related products for Origami™ Competent Cells | |
| 70627 | Origami™(DE3) Competent Cells |
| Product information | |||||||||||||||||||
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| Avoid freeze/thaw | Yes | ||||||||||||||||||
| Components |
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| Guaranteed efficiency | Guaranteed efficiency: > 2 x 106 cfu/µg | ||||||||||||||||||
| Store and ship information | |||
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| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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