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Merck KGaA
Frankfurter Str. 250
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Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
15 February 2012
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Origami™ B 5615 is a new T7Select® Phage Display System host strain closely related to the Origami host strain used for recombinant protein expression. Origami host strains are K-12 derivatives that have mutations in both the thioredoxin reductase (trxB) and glutathione reductase (gor) genes, which together greatly enhance disulfide bond formation in the cytoplasm. This new strain carries an ampicillin-resistant, medium–copy number plasmid, pAR5615, that expresses the native T7 capsid protein under the control of the lacUV5 promoter.
Genotype
F– ompT hsdSB rB– mB–) gal dcm lacY1 ahpC gor522::Tn10 (TcR) trxB::kan pAR5615 (ApR
Genotype
F– ompT hsdSB rB– mB–) gal dcm lacY1 ahpC gor522::Tn10 (TcR) trxB::kan pAR5615 (ApR
| Product information | |||
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| Declaration | Origami is a trademark of EMD Biosciences, Inc., Madison, WI USA | ||
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| Storage | ≤ -70°C | ||
| Ship |
Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Standard Handling |
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