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15 February 2012
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Origami™ 2 host strains are K-12
derivatives that have mutations in both the
thioredoxin reductase (trxB) and glutathione
reductase (gor) genes, which greatly enhance
disulfide bond formation in the E. coli
cytoplasm.
The Origami
2 strains are kanamycin sensitive; like the
original Origami strains, the gor mutation is still selected
for by tetracycline. To reduce the possibility of
disulfide bond formation between molecules,
strains containing mutations in trxB and gor are
recommended only for the expression of proteins
that require disulfide bond formation for proper
folding.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac+ lacIq pro] (DE3) gor522::Tn10 trxB pLysS (CamR, StrR, TetR)
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac+ lacIq pro] (DE3) gor522::Tn10 trxB pLysS (CamR, StrR, TetR)
| Related products | |
|---|---|
| 71344 | Origami™ 2 Competent Cells |
| 71345 | Origami™ 2(DE3) Competent Cells |
| 71431 | Origami™ 2 Competent Cell Set* |
| Related products for Origami™ 2(DE3)pLysS Competent Cells | |
| 71344 | Origami™ 2 Competent Cells |
| 71345 | Origami™ 2(DE3) Competent Cells |
| 71431 | Origami™ 2 Competent Cell Set* |
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| Components |
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| Guaranteed efficiency | 2 × 106 cfu/µg | ||||||||||||||||||
| Store and ship information | |||
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| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Licence Brookhaven | This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license. | ||
