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Phone: +49 6151 72-0
Fax: +49 6151 72 2000
15 February 2012
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Origami™ 2 host strains are K-12
derivatives that have mutations in both the
thioredoxin reductase (trxB) and glutathione
reductase (gor) genes, which greatly enhance
disulfide bond formation in the E. coli
cytoplasm.
The Origami
2 strains are kanamycin sensitive; like the
original Origami strains, the gor mutation is still selected
for by tetracycline. To reduce the possibility of
disulfide bond formation between molecules,
strains containing mutations in trxB and gor are
recommended only for the expression of proteins
that require disulfide bond formation for proper
folding.
Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139
ahpC galE galK rpsL F′[lac+ lacIq pro] gor522::Tn10
trxB (StrR, TetR)
| Related products | |
|---|---|
| 71345 | Origami™ 2(DE3) Competent Cells |
| 71346 | Origami™ 2(DE3)pLysS Competent Cells |
| 71431 | Origami™ 2 Competent Cell Set* |
| Related products for Origami™ 2 Competent Cells | |
| 71345 | Origami™ 2(DE3) Competent Cells |
| 71346 | Origami™ 2(DE3)pLysS Competent Cells |
| 71431 | Origami™ 2 Competent Cell Set* |
| Product information | |||||||||||||||||||
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| Components |
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| Guaranteed efficiency | 2 × 106 cfu/µg | ||||||||||||||||||
| Store and ship information | |||
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| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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