71089
One Step RT-PCR Kit
Convenient one-enzyme, single-buffer system for RT-PCR
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
15 February 2012
loading
This product has been discontinued.
We are offering One Step RT-PCR Master Mix Kit (Cat. No. 71978) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.
The One Step RT-PCR Kit will be available until stock is depleted. This kit will be replaced by the One Step RT-PCR Master Mix Kit (Cat. No. 71978-3), available April 30, 2008. More information will be available on this website soon. Please contact Technical Service for more information.
The One Step RT-PCR Kit* is designed for rapid, sensitive detection of gene expression in tissues and cells. The kit takes advantage of the properties of recombinant Thermus thermophilus (rTth) DNA Polymerase, which acts as both a thermostable RNA-dependent DNA polymerase and a DNA-dependent DNA polymerase. In a single reaction, cDNA is synthesized from input RNA through reverse transcription, followed by PCR amplification of the cDNA, without changing the buffer or adding reagents. The amplified DNA is normally analyzed by agarose gel electrophoresis.
Use of gene-specific primers, or oligo(dT) and one gene-specific 5′-primer, is recommended with the kit. Detection of a specific message requires approximately two hours.
Each kit provides sufficient reagents to perform 50 RT-PCR analyses. Positive Control RNA and Control Primers are also included.
*Manufactured by Toyobo and distributed by EMD Biosciences, Inc. Not available from EMD Biosciences, Inc. in Japan.
| Product information | ||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Components |
|
|||||||||||||||||||||||||||||
| Features and benefits |
|
|||||||||||||||||||||||||||||
| Declaration | Manufactured by Toyobo and distributed by Novagen. Not available from Novagen in Japan. | |||||||||||||||||||||||||||||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Dry Ice Only
Standard Handling |
||
