71091
NovaTaq™ Hot Start DNA Polymerase
Heat-activatable modified form of recombinant Taq DNA polymerase
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
15 February 2012
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NovaTaq™ Hot Start DNA Polymerase is a chemically modified form of Taq DNA polymerase that is inactive at ambient temperature. The enzyme provides improved specificity when compared to standard Taq DNA polymerase and can minimize the generation of nonspecific amplification products, such as primer-dimers and misprimed products. The enzyme must be activated by heat treatment (7–10 min at 95°C), after which thermal cycling can proceed. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning with the Novagen Perfectly Blunt®, AccepTor™, and LIC Vector Kits.
| Source | Recombinant Thermus aquaticus DNA polymerase expressed in E. coli |
| Concentration | 5.0 U/µl |
| Endonuclease | None detected |
| Exonuclease | None detected |
| Amplification effiency | Functional PCR |
| Storage | –20°C |
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| Unit definition | One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 72°C, in a reaction containing 25 mM TAPS (tris-[hydroxymethyl]-methyl-amino-propane-sulfonic acid, sodium salt), pH 9.3 at 25°C, 50 mM KCl, 2 mM MgCl2, 1 mM 2-mercaptoethanol, 0.2 mM dATP, dGTP, and dTTP, 0.1 µM [α-32P]dCTP, and 12.5 µg activated salmon sperm DNA in a volume of 50 µl. | |||||||||||
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| Declaration | Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA. | |||||||||||
| Store and ship information | |||
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| Storage | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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